$$\begin{array}{rlrl} 1&{_{\:\!\mathbf|}}\!\underline{\,~\neg \exists xPx~}\\[-5pt] 2&\big|~{_{\:\!\mathbf|}}\!\underline{\,~Pa~}\\[-5pt] 3&\big|~\big|~\exists xPx&\rm{{\exists}I}&\rm{2}\\[-5pt] 4&\big|~\big|~\bot&\rm{{\neg}E}&\rm{1,3}\\[-5pt] 5&\big|~\neg Pa&\rm{{\neg}I}&\rm{2{-}4}\\[-5pt] 6&\big|~\forall x\neg Px&\rm{{\forall}I}&\rm{5}\\[-5pt] \end{array}$$